Coding
IMPDH-C1/2

Part:BBa_K3308051

Designed by: Jemy Varghese, Ripal Sheth, Victor So, Harrison Green, Melissa Marciesky   Group: iGEM19_Pittsburgh   (2019-10-05)


Nested IMPDH C terminus- [IMPDH-1 C (1-35)]

This construct is the first half of the nested C-terminal of fast splicing intein IMPDH. It contain the first 35 amino acids of the intein's c-teminus and is directly attached to. the full intein half of intein TvoVMA, coded as [Tvo VMA C48 N]. The his tag upstream of the IMPDH split half.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None